2nd Year Biology Chapter 10 Test10. Biotechnology

Q1. Multiple choice questions (Attempt all · 10 × 1 = 10)

1. Biotechnology can be defined as the use of:
   1. Metals
   2. Microorganisms
   3. Animals
   4. Air
2. Fusion proteins for insulin production are combined with:
   1. Histones
   2. β-galactosidase
   3. DNA ligase
   4. RNA polymerase
3. The insulin gene is located on which human chromosome?
   1. Chromosome 7
   2. Chromosome 11
   3. Chromosome 5
   4. Chromosome 9
4. The size of the insulin gene is approximately:
   1. 5.0 Kbp
   2. 0.5 Kbp
   3. 1.5 Kbp
   4. 3.5 Kbp
5. The machine used in PCR to regulate temperature is called:
   1. Incubator
   2. Fermenter
   3. Autoclave
   4. Thermocycler
6. Kary B. Mullis was awarded the Nobel Prize in Chemistry in:
   1. 1983
   2. 1985
   3. 1990
   4. 1993
7. Restriction enzymes recognize specific sequences in DNA called:
   1. Replication sites
   2. Palindromic sequences
   3. Mutation sites
   4. Codons
8. DNA molecules with complementary sticky ends associate by
   1. covalent bond
   2. hydrogen bond
   3. ionic bond
   4. disulphide bond
9. The process by which bacteria protect their own DNA from restriction enzymes is called:
   1. Transcription
   2. Replication
   3. Methylation
   4. Translation
10. Which technique rapidly replicates specific DNA fragments without cloning?
    1. gel electrophoresis
    2. cDNA libraries
    3. genetic probe
    4. polymerase chain reaction