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Punjab (PTB) · Chapter test · Free

2nd Year Biology Chapter 8 TestBIOTECHNOLOGY

30 marks · 40 minutes · 21 questions. Covers: CHAP 20: BIOTECHNOLOGY.

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2nd Year Biology Chapter 8 Test

2nd Year · Biology · Punjab (PTB)

Name: ____________Roll No: ________Class/Section: ________Date: ________Total marks: 30Time: 40 minutes

Q1. Multiple choice questions (Attempt all · 10 × 1 = 10)

  1. Why has most gene research in humans focused on somatic cells instead of germline cells?

    1. Somatic cells are much larger

    2. Due to risks and ethical issues

    3. Somatic cells do not contain DNA

    4. Germline cells cannot take up foreign genes

  2. Identify the FALSE statement.

    1. Restriction enzyme is used for the production of RFLPs.

    2. DNA ligase enzyme is used to cut DNA, creating the sticky ends

    3. Reverse transcriptase enzyme is used for making cDNA from mRNA

    4. Electrophoresis means the separation of DNA fragments

  3. What are the identical copies of the gene of interest produced by bacterial clones called?

    1. Mutants

    2. Clones

    3. Hybrids

    4. Vectors

  4. Transgenic goats produce which human enzyme in their milk to fight diarrhea-causing bacteria?

    1. Amylase

    2. Lysozyme

    3. Pepsin

    4. Lipase

  5. Which technique is used for cloning of gene/DNA?

    1. RFLP analysis

    2. Polymerase Chain Reaction (PCR)

    3. Electroporation

    4. Gel electrophoresis

  6. Which of these can serve as ideal expression system for gene cloning?

    1. Bacteriophages

    2. Bacterial cells

    3. Plant cells

    4. Animal cells

  7. Which of the following is an example of traditional biotechnology?

    1. Polymerase Chain Reaction

    2. Gene deletion

    3. Fermentation

    4. DNA splicing

  8. What is the process used to distinguish between a "plain" plasmid and a "recombinant plasmid" called?

    1. DNA Cleavage

    2. Gel Electrophoresis

    3. Polymerase Chain Reaction

    4. Blue-White Screening

  9. Which host bacteria is used as a factory to produce Chain A and Chain B of insulin?

    1. Lactobacillus

    2. Escherichia coli (E. coli)

    3. Salmonella

    4. Bacillus subtilis

  10. What is an "Expression System"?

    1. A suitable host organism that expresses the gene of interest

    2. An enzyme that cuts target DNA

    3. A specialized laboratory device

    4. The sequence that marks the origin of replication

Q2. Short questions (Attempt any 6 of 8 · 6 × 2 = 12)

  1. How have scientists created malaria-resistant mosquitoes in the laboratory?

  2. Why are bacterial cells preferred as an ideal expression system?

  3. What is the primary function of Polymerase Chain Reaction (PCR)?

  4. How are genetically modified viruses used in gene therapy?

  5. How are host cells transformed to produce both insulin chains?

  6. What is DNA Ligase and why is it called molecular glue?

  7. Why do scientists need to perform "Screening of Clone Library"?

  8. Define Denaturation in PCR.

Q3. Long questions (Attempt any 2 of 3 · 2 × 4 = 8)

  1. Investigate careers that require an understanding of biotechnology and genetic engineering.

  2. Describe the methods for the introduction of human DNA into bacteria.

  3. Write a detailed note on restriction enzymes.