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Federal (FBISE) · Chapter test · Free

2nd Year Chemistry Chapter 19 Test19. Chromatography

30 marks · 40 minutes · 21 questions. Covers: 19. Chromatography.

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2nd Year Chemistry Chapter 19 Test

2nd Year · Chemistry · Federal (FBISE)

Name: ____________Roll No: ________Class/Section: ________Date: ________Total marks: 30Time: 40 minutes

Q1. Multiple choice questions (Attempt all · 10 × 1 = 10)

  1. Shorter retention times are associated with:
    1. Polar components with lower boiling points
    2. Non-polar components with high boiling points
    3. Metals
    4. Solvent molecules only
  2. The stationary phase in chromatography functions mainly as:
    1. A solvent
    2. An adsorbent
    3. A lubricant
    4. A catalyst
  3. In chromatography, the stationary phase may be ______ supported on a solid.
    1. Solid or liquid
    2. Liquid or gas
    3. Solid only
    4. Liquid only
  4. In a scenario where a person may have died from a drug overdose at a crime scene, how can mass spectrometry contribute to determining the cause of death?
    1. Mass spectrometry can detect the presence of a toxin but not specify which toxin.
    2. Carpet fiber analysis can be utilized to match fibers found on the victim.
    3. Tissue samples can be examined to identify both the presence and quantity of toxins.
    4. Mass spectrometry can ascertain the presence of a toxin but not quantify it.
  5. Which type of stationary phase is better for separating closely related compounds?
    1. Large particles and viscous mobile phase
    2. Small particles and low viscosity mobile phase
    3. Large particles and low viscosity mobile phase
    4. None of these
  6. Which of the following is an advantage of TLC?
    1. Very expensive
    2. Difficult to perform
    3. Simple, fast, and inexpensive
    4. Not useful in industry
  7. What is the distance that the solute moves while undergoing one partition?
    1. Retention distance
    2. Distribution constant
    3. Plate height
    4. Column packing length
  8. The property of a solvent that ensures proper flow rate and peak shape is:
    1. Color
    2. Viscosity
    3. Transparency
    4. Odor
  9. A solvent used in chromatography must be:
    1. Chemically reactive
    2. Easily evaporated
    3. Light absorbing at UV range
    4. Chemically stable and non-degrading
  10. The level at which solvent reaches as it moves up the paper is called:
    1. Solvent front
    2. Baseline
    3. Origin line
    4. Stationary point

Q2. Short questions (Attempt any 6 of 8 · 6 × 2 = 12)

  1. Why should solvents not absorb UV light in UV detection methods?
  2. Give two applications of chromatography.
  3. Define thin layer chromatography (TLC).
  4. How do polar compounds behave on TLC plates compared to non-polar compounds?
  5. Define Rf (retardation factor).
  6. What causes colors to separate during chromatography?
  7. State one use of TLC in document analysis.
  8. How do consistent Rf values help in substance identification?

Q3. Long questions (Attempt any 2 of 3 · 2 × 4 = 8)

  1. Explain the interpretation of Rf values in chromatography with a diagram.
  2. Explain the working principle of mass spectrometry in detail.
  3. Explain the process of chromatography with the help of a diagram.